PROTECTIVE EFFECT OF ALPHA-LIPOIC ACID AGAINST EXPERIMENTAL CARDIOTOXICITY INDUCED BY ISOPROTERENOL IN ADULT MALE ALBINO RATS: A HISTOLOGICALAND BIOCHEMICAL STUDY
Abir Oueida El Sadik
Anatomy Department, Faculty of Medicine, Cairo University
Introduction: Isoproterenol (ISO) has been found to induce myocardial toxicity through several mechanisms including oxidative stress that lead to myocardial necrosis. Alpha Lipoic acid (LA) has an antioxidant role that neutralizes the free radicals released during the oxidation process and exerts anti-inflammatory effects.
Aim of work: to investigate the possible protective role of LA on cardiac toxicity induced experimentally in adult male albino rats with ISO treatment.
Material & Methods: the present study was implemented on forty adult male Albino rats divided into 4 equal groups: group I (control group), group II (LA treated group) received LA at a dose (100 mg/kg body weight) orally daily for 7 days by gavage, group III (ISO treated group) received ISO (85 mg/kg body weight, subcutaneously) on the fifth and sixth days and group VI (LA and ISO treated group) received LA (100 mg/kg body weight) orally daily for 7 days by gavage and in addition received ISO (85 mg/kg body weight, subcutaneously) on the fifth and sixth days. The animals were sacrificed at the end of the experiment, blood samples were collected for biochemical investigations and the left ventricles were excised and prepared for histological and ultrastructural study.
Results: Administration of LA before ISO significantly prevented ISO-induced elevation of serum cardiac marker enzymes; creatine kinase (CK) and its myocardial isoenzyme (CK-MB). Light and electron microscopic findings of the heart pretreated with LA revealed a well preserved normal histology of cardiac muscle with minimal evidence of myocardial injury when compared to ISO-treated hearts.
Conclusion: LA was suggested to have the ability to protect the cardiomyocytes against the cardiotoxic effect of ISO in adult male albino rats.