SIOLOGICAL EFFECT OF SELENIUM& GREEN TEA ON NEPHROTOXICITY RATS
Hewaida A.E. Fadel; Essayed A. Hassanin*
Department of Nutritional Chemistry & Metabolism (Physiology and *Biochemistry), National Nutrition Institute
Various environmental agent such as (chemical pesticides, solvents & similar materials), animal venom, certain plants and some drugs are nephrotoxic by producing free radicals such as (O2, RO2, OH, NO2, NO) which can cause kidney damage and dysfunction by starting chain reactions that damage kidney cells. Antioxidants are molecules capable of preventing these chain reactions by removing free radicals intermediates and inhibit other oxidation reactions by oxidized themselves. Supplementation with antioxidants could be useful in reducing nephrotoxicity.
Aim of the work: The present study was a trial for investigating the physiological effect of supplementation with Selenium& Green tea as natural antioxidant extracts in nephrotoxic rates.
Material & Methods: 32 adult male Sprague-Dawley rats (150-200 g) were divided into four groups (8 rats/group). Group (1): were fed on standard diet (S.D.), as a negative control group. The other rats were injected intraperitoneally with a single dose of Cis-diammine dichloride Platinum II (CDDP) for inducing renal dysfunction (2.5 mg/Kg) then they were divided into three groups. Group (2): fed on (S.D.) nephrotoxic group as a positive control group. Group (3): fed on (S.D.) + Selenium (0.6 mg/Kg body weight/day). Group (4): fed on (S.D.) + Green Tea Extract (100 mg/kg body weight/day). The experimental period was four weeks, results were statistically analyzed.
Results: The results proved that group of nephrotoxicity rats supplemented with Selenium showed significant increase in food intakes (FI), while there was significant decrease in (FI) for Green tea extract group. Selenium& green tea groups showed significant increase in body weight gain (BWG) and food efficiency ratio (FER) compared with nephrotoxic group. Rats supplemented with Selenium and Green tea showed significant increase in serum vitamin E &kidney glutathione compared with positive control group. The results showed that nephrotoxicity rats supplemented with Selenium and Green tea showed significant reduction in serum urea nitrogen, creatinine, uric acid, nitric oxide, serum sodium while, serum potassium was significantly elevated compared with nephrotoxic group. Best results in histopathological examination of kidney were in Selenium group.
Conclusion: These results suggest that the use of selenium& green tea could be beneficial in improving kidney functions in nephrotoxicity rats.