DETECTION OF CRYPTOSPORIDIOSIS IN CANCER PATIENTS IN MENOUFIYA GOVERNORATE WITH IMPACT OF STORAGE MEDIA, TIME AND CONCENTRATION ON CRYPTOSPORIDIUM OOCYST DETECTION BY POLYMERASE CHAIN REACTION
Salwa A. Shams El-Din
Parasitology Department-Faculty of Medicine-Menoufiya University-Egypt.
Background: Cryptosporidium parvum is a common protozoan pathogen with worldwide distribution. It localizes on the intestinal cells with prolonged diarrhea in immunocompromised patients. Objectives: The aim of this study was to estimate the prevalence of enteric cryptosporidiosis in patients with malignancies and the effects of different storage durations and media on parasite detection by polymerase chain reaction.Material & Methods: In this cross-sectional study stool samples were collected from 71 pateints with different malignancies. Stool samples from control patients (15) free of malignancies were also examined. All of the specimens were examined for the oocysts of C. parvum by modified Ziehl Neelsen (MZN) staining technique and polymerase chain reaction for C. parvum DNA. Different solvents were used for preservation of C. parvum oocysts for different durations and samples were examined by polymerase chain reaction. Findings: Cryptosporidium infection was detected in 13 patients (18.3%) by MZN stain and 29 patients by PCR (40.8%). One infected person from control group was recorded by MZN stain and PCR. Fresh samples were found all positive by PCR with all media tested while PCR detection results of old samples with different preservatives were variable.Conclusion: The incidence of Cryptosporidium infection was high among patients with malignancy and successful PCR amplification from oocysts is dependent on the temperature, duration and media used for adequate sample storage.