PROTECTIVE EFFECT OF VITAMIN E ON TESTICULAR CHANGES INDUCED BY CHRONIC SUPPLY OF A LOW ALUMINIUM DOSE IN YOUNG RATS
Manal E. El-Sawaf
Anatomy department, Faculty of medicine, Tanta University
Background: Aluminium salts (Al) are substances found naturally in water and food or present in the components of drugs. Toxic effects of high doses of Al on testicular tissue have been monitored in previous researches. Al also was proved to be accumulated in target organs on long term ingestion even on its administration in very low non-toxic doses.Aim of the work: To study the possible damaging effects of chronic administration of a low dose of Al on testes of young rats and the possible protective role of vitamin E .Material & Methods: Twelve male rats aged three weeks were used in this study and were divided into three groups; group (I) was served as control, group (II) was given aluminium chloride (AlCl3) orally while group (III) was given both AlCl3 and vitamin E . The treatments were given daily for 18 weeks after which rats were weighed before being sacrificed. Testes were collected weighed and then processed for light microscopic examination.Results: Testes collected from group (II) rats showed significant reduction of absolute and relative weights compared to that of the control. Histomorphometric study revealed decreased diameter of seminiferous tubules with decreased height of lining epithelium. Histological examination demonstrated degenerative changes of spermatogonia and necrotic spermatozoa. Tunica albuginea was thickened, lamellated and irregular with subcapsular cavities and dilated congested blood vessels. Testes collected from group (III) rats showed higher absolute and relative weights than group (II). Diameter of seminiferous tubules and height of lining epithelium showed increased values than group (II). Restoration of spermatogonia and tunica albuginea were also seen.Conclusion: Aluminium chloride induced testicular damage in chronic use of low doses which was alleviated by the concomitant use of vitamin E.