EVALUATION OF EARLY EFFICACY OF MEFLOQUINE ON EGYPTIAN S. MANSONI STRAIN IN INFECTED MICE
Maysa Ahmad Eraky1, Reda M. El-Badawi2 and Olfat Ali Hammam3
Departments of Parasitology1 and Gastroenerology, hepatology and Infectious Diseases2, Faculty of Medicine, Banha University Department of Pathology3, Theodore Bilharz Research Institute
The treatment and control of schistosomiasis relies on a single drug, praziquantel; hence, there is a need to develop new antischistosomal drugs. The purpose of the present study is to assess the parasitological and histopathological changes in mice infected with Egyptian S. mansoni strain at different stages of infection (juvenile, acute and chronic infection) after treatment with mefloquine (200mg/kg). Mice were infected with 80±10 S. mansoni cercariae, then treated with a single dose of mefloquine at 21, 49, 98 days post infection. One week post treatment mice were sacrificed. Results: Mefloquine treatment showed a marked reduction of total immature worm load in immature infection (94.11%). In chronic infection, the total worm load reduction was 91.68% with increase in the % uncoupling (100%), while in acute infection no detectable reduction in total worms, with increase of % uncoupling (26.8%) was observed. This worm load reduction was acompanied by reduction of the hepatic and intestinal tissue egg load in both acute and chronic infection, which is more in chronic infection. Analysis of the oogram pattern revealed a significant reduction of the immature egg stages associated with significant increase in the mature and dead egg stages in acute and chronic infection, however, the increase in mature and dead stages was more marked in chronic infection. Histopathological examination of livers of infected untreated and mefloquine treated mice, revealed that, there was slight significant reduction in granuloma volume (diameter), in both acute and chronic stages. Conclusion: The results indicate that, the smaller dose of mefloquine (200mg/kg) shows strong antischistosomal properties mainly on Egyptian juvenile and chronic infections than in acute infection which may need a larger dose to obtain the lethal effect.