COMPARATIVE STUDY OF THE EFFECT OF GREEN TEA, GARLIC AND ONION ON THE ISLETS OF LANGERHANS OF DIABETIC RABBITS INDUCED BY ALLOXAN
Morsy A. Abo-Elgoud
Anatomy Department, Faculty of Medicine, Tanta University
Background: Diabetes mellitus is a complex disorder that is characterized by hyperglycemia resulting from malfunction in insulin secretion or insulin action. Despite the presence of known antidiabetic medicine, diabetes and the related complications continued to be a major medical problem. Recently, green tea, garlic and onion have been reported to be useful as antidiabetic agents. Aim of the work: This study was conducted to clarify the feasibility of green tea, garlic and onion as assisting agents in treatment of diabetes and comparison between them.Material and methods: In this study 25 male albino rabbits each weighing 1-1.5 kg were used and classified into two groups. The first group (five rabbits) used as –ve control; the second experimental group (twenty rabbits) was injected intraperitonealy with single dose of alloxan (50 mg/kg). The experimental group was classified into four subgroups (five rabbits for each). First subgroup used as diabetic +ve control, second, third and fourth subgroups received green tea (100mg/kg/BW), garlic (0.4 g/100 g BW) and onion (0.4 g/100 g BW) orally once daily on the 3rd day after alloxan injection for 15 days. At the end of the experiment, all fasted rabbits were sacrificed and their blood samples were collected for measuring blood sugar level and serial sections were prepared from their pancreatic glands and then examined by light microscope.Results: After induction of diabetes, the islets of Langerhans revealed significant architectural disarray and they were damaged and shrunken. The islets cells showed degeneration of their cytoplasm, loss of their cellular membrane, pyknotic nuclei and necrosis. The connective tissue membrane between the islets of Langerhans and pancreatic acini was destructed. Also the number of -cells and the size of islets area were decreased. The quantitative immunohistochemical studies demonstrated significant decrease in the intensity of --cells anti-insulin antibody reaction. On the other hand, these results illustrated that donation of green tea, garlic, and onion apparently reduced the n-cells damage and the number of --cells and the size of islets area were increased. Also significant increase in the intensity of anti-insulin antibody positive reaction was noticed.Conclusion: The present study revealed that green tea, garlic and onion administrations are effective in protecting the islets of Langerhans against diabetes. This study proved that the green tea was the best then garlic and lastly onion.