EFFECT OF DERMAL APPLICATION OF LINDANE ON THE TESTICULAR TISSUE OF RAT: A HISTOLOGICAL AND ULTRASTRUCTURAL STUDY.
Raeesa Abdul Twab Mohammed
Department of Histology and Anatomy-Faculty of medicine-King Saud University
Objective: Lindane, the γ isomer of hexachlorocyclohexane (HCH), is one of oldest synthetic pesticides still in use worldwide. Numerous reports have shown that this pesticide adversely affects reproductive function in animals. Although the pathogenesis of reproductive dysfunction is not yet fully understood, recent reports indicate that lindane can directly inhibit adrenal and gonadal steroid genesis.The aim of this research was to find out the effect of lindane on testicular tissue in adult male rats.Methods: Adult male rats received lindane formulated in 5% aqueous carboxymethylcellulose as dermal application on the skin 6 hours per day for 5 consecutive days per week for 7 weeks at a dose to 100mg/ kg of body weight. Twenty-four hours after the last treatment, the rats were killed using cervical dislocation. Testes were removed and processed for both light and electron microscopic study. Hormonal analysis, to determine the blood level of testosterone, FSH and LH, was done.Results: Light microscopic study revealed arresting spermatogenesis and degeneration of spermatogenic cells in addition to depletion of the interstitial (Leydig) cells. Histological investigation by electron microscope confirmed the spermatogenic cells degeneration in the form of rarefaction of the cellular cytoplasm and degeneration of their nuclei. The interstitial tissue appeared empty of Leydig cells. But the sertoli cells showed no changes. Hormonal analysis revealed decreasing level of testosterone, FSH and LH hormones.Conclusion: Dermal application of lindane, as happen in agriculture, can induce histological changes in the testes.