EVALUATION OF THE PROTECTIVE ROLE OF SILDENAFIL CITRATE ON CISPLATIN INDUCED -NEPHROTOXICITY IN ADULT MALE ALBINO RATS: HISTOLOGICAL, BIOCHEMICAL, HISTOCHEMICAL AND HISTOMORPHOMETRIC STUDY
Ayman Abo El Enein Rizk - Ahmed Galal Motawie
Anatomy and Embryology Department- Faculty of Medicine- Cairo University
Background: Nephrotoxicity is considered the most important side effect which limits cisplatin therapy in various diseases. It is might be due to oxidative stress, decreased renal blood flow and reduction in the glomerular filtration. Sildenafil citrate is used for treatment of erectile dysfunction but its effect in ameliorating cisplatin nephrotoxicity was not yet clearly studied. So the present work aimed to evaluate the protective role of Sildenafil Citrate against cisplatin-induced nephrotoxicity in adult male albino rat. Materials and Methods: 24 adult male albino rats were divided into 4 groups (6 rats each); Group I: control. Group II: sham control. Group III: cisplatin treated group and Group IV: Sildenafil and Cisplatin treated group. At the end of the experiment, the kidney of each animal was excised, trimmed and prepared for histological, histochemical and immunohistochemical study. Blood samples were obtained to evaluate the kidney functions. Results: kidney sections of group III showed marked degenerative changes in the proximal convoluted tubules; vacuolations, exfoliation of the lining epithelium, cast formation and interstitial hemorrhagic exudate. There was marked elevation of serum creatinine and urea with significant increase in NO and decrease in GSH concentrations in the kidney tissue and weak PAS reaction. Treatment with Sildenafil Citrate in Group IV offered marked improvement in the renal structure, kidney function tests and other parameters. Conclusion: Sildenafil Citrate could protect the kidney against Cisplatin-induced nephrotoxicity in adult male albino rat.
Conclusion: Omega 3 could ameliorate the toxic effect of aspartame on the cerebellum of adult male albino rat.