EXPERIMENTAL STUDY ON THE CYTOTOXICITY OF DIFFERENT SIZES GOLD NANOPARTICLES (10, 50NM) ON CARDIAC MUSCLE OF ADULT MALE ALBINO RATS.
Feryal F. Ibrahim, Ehab M. El-Zwawy, Fardos, S. Karawya, Abeer G. Ahmed, Amira H. El-Trawy.
Department of Human Anatomy and Embryology with department of Histology and Cell Biology, Faculty of Medicine, University of Alexandria.
Objective: Environmental exposure to nanomaterials is inevitable as nanomaterials became part of our daily life. Nanoparticles are widely distributed in air, cosmetics, medicine and even in food due to rapid expansion of nanoparticle use in various fields, including drug and gene delivery, biosensors, cancer treatment and diagnostic tools. Also commercial products containing nanoparticles released on the market is rapidly increasing, and as a result, nanotoxicity research is gaining attention. Aim: In the present study we examined the possible histological alterations in the cardiac muscle after administration of gold nanoparticles (AuNPs) in different sizes(10 nm and 50 nm) in an attempt to cover and understand the toxicity and the potential role of AuNPs in the therapeutic and diagnostic applications. Material & methods: to investigate the particle size effect of (AuNPs) on cardiac muscle, a total of 20 healthy adult male albino rats were used. The animals were injected intraperitoneally with 200 μl AuNPs (10,50 nm) for 7 days. Results: In comparisonwith respective control rats, exposure to AuNPs induced alterations in the cardiac muscles which were summarized mainly as destruction of cardiomyocytes and its widely separation by edema, loss of striation due to presence of contraction bands, irregular nucleus surrounded by wide perinuclear space, swollen and abnormal shape mitochondria,dilated T- tubules and disruption of intercalated discs. Conclusion: AuNPs induced evident size dependent damage in rat cardiac muscle. This damage was more severe with 10 nm particles than with 50 nm particles.