TOTAL ANTIOXIDANT CAPACITY IN FOLLICULAR FLUID IN AGONIST AND ANTAGONIST PROTOCOL AND ITS RELATION TO INTRA CYTOPLASMIC SPERM INJECTION (ICSI) OUTCOME
Ahmed Salah El Gheriany, Mohamed Salah Eldeen Abd Raboh, Sherif Salah Gaafar
Obstetrics and Gynecology Department, Faculty of Medicine, Alexandria University
Background and objective: Follicular Fluid contains many markers that can affect the ICSI outcome, oocyte and embryo quality. Measuring of total antioxidant capacity (TAC) in the follicular fluid could provide a good marker for evaluating the quality of oocytes, and since the procedure of measuring TAC not time consuming it appears to be a good tool. It is not known whether TAC is different between the GnRh agonist and antagonist protocol, which is the aim of the current study.
Methods: This study was performed on 40 infertile couples undergone ICSI, divided into 2 groups 20 patients in every one managed by agonist and antagonist protocols. During pick up, pooled follicular fluid was collected for TAC assay using ELISA TAC fast track kit.
Results: As regard to TAC, in agonist group it ranged between 45.3-369.5 with mean±SD 236.18±90.637 while in antagonist group it ranged between 84 and 381.1 with mean±SD 227.22±89.493. There was no statistically significant differences between the two groups while p = 0.755 (significant level at p < 0.05). Also, as regards TAC in all pregnant cases in both groups it ranged between 45.3 and 348.9 with mean±SD 200.356±94.007 while in non-pregnant cases in both groups it ranged between 84 and 381.1 with mean±SD 252.596±80.839. There was no statistically significant differences as regard TAC between the pregnant and non-pregnant cases while p = 0.280. Also, there was no statistically significant difference in Oocyte fertilization and cleavage rates (p = 0.710), and Pregnancy and multiple pregnancy rates (p = 0.356) between the agonist and antagonist groups.
Conclusion: There is no statistically significant difference regarding total antioxidant capacity (TAC) in follicular fluid in patients undergone ICSI using long agonist protocol and antagonist protocol p = 0.755. Further studies are required in the future with more cases to confirm the relation of GnRH analogues with antioxidant and its implication in follicular growth and ICSI outcome.