SOYBEAN SPROUTS AND DRIED BLACK PLUM ANTAGONIZE THE DELETERIOUS EFFECT OF OXIDIZING AGENTS IN RATS
Maha H. Mahmoud
Food Science & Nutrition Dept., National Research Centre, Dokki, Cairo, Egypt.
Background: Recently, more attention is paid to oxidative stress and its dangerous role in degenerative diseases such as cancer and atherosclerosis. Many researches are directed now towards the protective role of dietary antioxidants, specially polyphenols. Aim: This study aims to evaluate the efficiency of polyphenol-rich plants namely; soybean sprouts and dried black plum to antagonize or even minimize the hazardous effect of oxidizing agents like sodium nitrite which is one of the widely used food additives and its use can not be avoided up till now. Methods: A model of increased oxidative stress was induced in rats by sodium nitrite then, the protecting antioxidant effect of soybean sprouts and dried black plum was evaluated. Four groups of rats were included in this study as follows; a control group was given the control diet, another group was fed on nitrite containing diet as 7000 ppm, a third group was given the nitrite containing diet + 20% dried soybean sprouts and the fourth group was given the nitrite containing diet + 20% dried black plum. The experiment lasted for four weeks. Food intake, body weight gain and FER were recorded. The following biochemical parameters that reflect the oxidation state were determined: SOD in erythrocytes, G6PDH in whole blood, plasma lipid peroxidation product as malondialdehyde, plasma vitamin E, blood methemoglobin and blood hemoglobin. Results: Data obtained from this study revealed that, there was a significant decrease in food consumption, body weight gain and FER of the nitrite fed group. Also, there was a significant decrease in blood hemoglobin, RBCs SOD and plasma vitamin E, while there was a significant increase in blood methemoglobin, the plasma lipid peroxidion product; malondialdehyde and blood G6PDH for rats fed on nitrite containing diet. Most of these parameters were normalized by addition of soybean sprouts and dried black plum. Conclusion: the use the antioxidant polyphenolic-rich food items; soybean sprouts and dried black plum can antagonize or even minimize the deleterious effect of sodium nitrite in rats. Thus, it is recommended that these two items should be more consumed or at least included in the diet of all people specially those who consume more foods with additives or those who are more subjected to oxidizing agents.