RAPID AND ACCURATE TECHNIQUES FOR LABORATORY DIAGNOSIS OF BOVINE VIRAL DIARRHEA
Iman M. Bastawecy; Omayma M. El-Desawy; W.F. Aly;Samya Sayed Abd EL-Nabi and Fatehia M. Mohamed
Dept. of virology, Animal Health Research Institute, Dokki, Cairo.
Bovine viral diarrhea virus (BVDV) is present in most cattle-producing countries. Detection and elimination of persistently infected (PI) cattle, along with vaccination, are necessary for control of BVD in cattle herds. In the present study, rapid and accurate techniques for laboratory diagnosis of BVD were used. Commercial antigen capture enzyme linked immunosorbent assay (ELISA) Kit , Staphylococcus aureus protein A-coagglutination test (SPA-COAT), negative staining electron microscopy (EM) and immunoelectron microscopy (IEM) were used as rapid tests for detection of BVD viral antigen in serum of PI and acutely infected cattle. Commercial BVD viral antigen capture ELISA for detection of non-structural protein 2-3 and EO was proved to be sensitive and specific, good for laboratories handling large numbers of specimens on daily basis to screen whole herds in less than 4 hours. SPA-COAT is rapid, sensitive, economic, read with naked eye and suitable for laboratories without requirements for instrumentation and technical training needed for ELISA. Negative staining EM and IEM remains a gold standard for antigen detection and confirmation for presence of pestiviral particles although requires expensive equipment and skilled personnel.