ANTICLASTOGENIC EFFECTS OF BARLEY AGAINST MUTAGENICITY OF CYCLOPHOSPHAMIDE AND CLOMIPRAMINE ON GERM CELLS OF MICE
Rokaya H. Shalaby, Fatma A. Sallam, Thanaa M. Bader and *Amal A. Selim
Zoology Department, Women’s College for Arts, Science and Education, Ain Shams University – *Um Alqura University, Biology Department Faculty of applied science (girls)
The aim of the present study was to study the possible modulatory anticlastogenic effects of pre treatment and post treatment with barley on germ cells (sperms) of mice treated with cyclophosphamide (CP) or clomipramine (CL)246 male albino mice, were divided into seven main groups (6 per each group or sub group ) treated as follows: control group, CP group (included two doses 5 mg/kg and 40 mg/kg. for 1, 3 and 15 days), clomipramine group (included two doses 25 mg/kg. and 75 mg/kg. for 7 days), barley treatment groups with two doses of 30 and 60 gm/day barley for 28 days include two main subgroups, pre treatment and post treatment groups. Mice were injected with CP and CL intraperitonealy (IP). They received barley by gavages. Animals were sacrificed to assay sperm shape abnormalities of treated animals. The most important result obtained in the present study was that CP or CL increased the percentage of sperm shape abnormalities by increasing dose and time. This percentage was decreased when barley was given before and after CP or CL. The reduction was very highly significant (P< 0.001) when compared with the control group. These results suggest that pre and post treatment with barley might increase the intracellular content of barley (β-glucan and phenolic compounds), thus intensifying the protection against damage induced by free radicals, reactive oxygen species (ROS), which may react with protein and DNA producing these abnormalities. From these findings it is suggested that barley may be advised before and during chemotherapy of malignant tumors.