POSSIBLE PROTECTIVE EFFECT OF GLUCOSAMINE SULFATE AGAINST ENDOTOXIN-INDUCED UVEITIS IN ALBINO RATS. A HISTOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY.
Dina Helmy Abdel-Kader and Safinaz Salah Eldin Sayed.
Histology Department, Faculty of Medicine, Cairo University.
Background & aim: Among all ocular inﬂammatory diseases, uveitis is a potent vision-threatening intraocular condition that can result in total blindness, however, its etiology is not, so far, fully understood. Immunosuppressants including steroids, used to control inflammation, causes serious side effects, including the threat of glaucoma and cataract. Glucosamine sulfate (GS) is a naturally occurring sugar that exerts immunosuppressive effects in vitro and in vivo. This study aimed at investigating the possible protective effect of GS against endotoxin-induced uveitis (EIU) in rats.Material and methods: EIU was induced in male albino rats by single subcutaneous injection of 200 Mg lipopolysaccharide (LPS). GS was administered intraperitoneally either at the same time or 30 minutes after LPS injection. Rats were killed 24 hours after LPS treatment. The eyes were enucleated and processed for H & E staining and immunostaining using iNOS and NFκb antibodies.Results: EIU was manifested within the CT in the base of the ciliary body as massive inflammatory cellular infiltration after 24 h from LPS injection. Treatment with GS ameliorated the inflammatory manifestations specially when administered concomitantly with LPS. A statistically significant increase in iNOS immunostaining was detected within the CT of the ciliary body and iris 24 h from LPS injection. This was evidently decreased when GS was given with LPS. NFκb immunostaining showed a statistically significant increase in the outer nuclear layer of the retina after 24 h from LPS injection that was markedly decreased especially when GS was given 30 min after LPS injection.Conclusion: GS can modulate the inflammatory changes of EIU in rats.