RIBOTYPING OF BLASTOCYSTIS ISOLATES FROM SYMPTOMATIC AND ASYMPTOMATIC HUMAN HOSTS IN EGYPT
Mohamed H. Fayad1, Anhar A. Mohamed1, Hanan H. Kamel1, Najmaldeen H.A.Algoul2
Department of Parasitology, Faculty of Medicine, Ain Shams University, Egypt1 and Department of Parasitology, Faculty of Medicine, Elfateh University, Libya2.
Blastocystis hominis is one of the most common protozoan parasites of human intestinal tract. The genetic diversity would be a powerful tool for identification and classification of B. hominis subtypes. In this study, 45 Blastocystis isolates, 40 from symptomatic and 5 from asymptomatic human hosts, were analyzed genetically by ribotyping; restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified small subunit SSU rDNA. The amplification products were digested with three restriction enzymes; AluI, HinfI and RsaI. Analysis of the riboprints revealed that all isolates belonged to subtype 3 (ribodeme 2), reinforcing the hypothesis of its human origin. Also there was no correlation between this subtype and pathogenicity of the parasite.