Th1, Th2 AND THE Th1:Th2 CELL RATIO IN NORMAL PREGNANCY AND PREECLAMPSIA
Mohamed Hasan Mostafa1 and Nashwa A. Abdel Kader2
1Department of Obstetrics and Gynecology, Faculty of Medicine, Cairo University
2Department of Clinical Pathology, Faculty of Medicine, Cairo University
This study was done to compare pregnancy-induced changes in T-helper derived cytokines in normal and preeclamptic pregnancies. Peripheral blood mononuclear cells were collected from 30 normal pregnant women in the third trimester and 32 women with preeclampsia, in addition to 20 nonpregnant women as a control group. T lymphocytes subsets were detected and enumerated. CD4+ T lymphocytes were identified using flow cytometry. CD4+TNF-α+IL-4- cells are identified as Th1 cells and CD4+TNF-α-IL-4+ cells as Th2 cells. The normal pregnancy and preeclamptic groups were matched for gestational age and fetal birth weight after delivery. The percentage of Th1 cells was significantly decreased in normal pregnant women in third trimester than in nonpregnant women (p = 0.003). The percentages of Th2 cells in third trimester pregnant subjects were rather high but not significantly higher than in nonpregnant subjects (p = 0.321). The ratio of Th1:Th2 in third trimester pregnant subjects was significantly lower than in nonpregnant women (p = 0.003). In preeclamptic patients, the percentage of Th1 cells (p = 0.049) and the Th1:Th2 cell ratio (p < 0.001) were significantly higher than in third trimester normal pregnant women. On the other hand, the percentage of Th2 cells in preeclamptic patients was significantly lower than in normal late pregnant women (p < 0.001). This study suggests some sort of inflammatory activity in preeclamptic patients; evident from predominance of Th1 cells in preeclampsia contrary to the predominance of Th2 cells in normal pregnancy.