ASSESSMENT OF CD34 PROGENITOR CELLS IN FRESH AND CRYO-PRESERVED UMBILICAL CORD BLOOD
Magdy A. EL-Barbary, Alaa El-Din. S. Abd El-Hamid*, Magda I. Mohamed, Noha M.Y. Abo-Gresha
Departments of Physiology and *Clinical Pathology, Faculty of Medicine, Suez Canal University, Egypt
Background: Recent researches documented that hematopoietic stem and progenitor cells in cord blood have a more potent proliferative ability than those derived from other sources and the effect of cryopreservation is variable up on these separated cells.
Aim: to determine the effect of cryopreservation on the quality and quantity of CD34+ve hematopoietic progenitor cells isolated from umbilical cord blood (UCB).
Methods: The study was carried out on 24 UCB samples at the end of full term vaginal deliveries. CD34+ cells were separated and purified by Immunomagnetic separation technique (MACs). Half of UCB samples were freshly assessed quantitatively and qualitatively and the remaining samples were cryopreserved. UCB samples were assessed for CD34+ cells count and viability before and after cryopreservation.
Results: Fresh CD34+ cell yield was 3±1.71 x 105/ml and its viability percentage was 87.08±4.75%. After cryopreservation, CD34+ cell count was 2.08±1.64 x 10+/ml and its viability percentage was 78.33±5.25%. The quantity and the quality of the CD34+ cells separated from freshly processed UCB was significantly higher compared to the quantity and the quality of the cells separated from cryopreserved UCB (p < 0.001).
Conclusion: Cryopreservation affects CD34+ progenitor cell count and viability to some extent.