POTENTIAL ANTIOXIDANT VALUE OF GARLIC ACID IN AMELORATING THE ADVERSE EFFECT OF ACRYLAMIDE ON ALBINO MALE ADULT RAT TESTIS. A HISTOLOGICAL, IMMUNOHISTOCHEMICAL AND BIOCHEMICAL STUDY
Ahmed H. Bayoumi1, Mousa, A.A.2 and Mohamed Dief Allah Abdelmoneam1
1Department of Anatomy and Embryology, Faculty of Medicine, Cairo University
2Department of Anatomy and Embryology, School of Medicine, Newgiza University (NGU)
Background: Acrylamide (ACR) can form in the high-temperature fried carbohydrate-rich foods providing a serious health risk and testicular injury is a possibility.
Aim of work: The purpose of this research was to perceive if garlic had protective impact against ACR-induced testicular injury.
Methods and materials: There were four groups of forty Sprague Dawley adult males rats, were used in the current study (10 rats each): Control, Sham control, ACR-treated, and acrylamide and garlic- acid treated groups.
Histological (H&E), immunohistochemical (caspase 3 and Proliferating Cell Nuclear Antigen “PCNA”) and testosterone levels, as well as superoxide, dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), histomorphometry in addition statistical analysis, stood executed.
Results: Deteriorated seminiferous tubules, vacuolations, and fluid exudates between tubules were seen in the ACR-treated group. Increased caspase 3 expression and MDA mean value and reduction in PCNA, GSH and SOD were seen in the same group. The tubules in the garlic-treated group were well-structured. Reduction in caspase 3 expression and MDA mean value as well as, elevation in PCNA, GSH and SOD were observed in the same group.
Conclusion: The testis alterations caused by ACR were due to the oxidative stress and there are marked improvement when treated with Garlic acid, which has ameliorative effects.
Key words: acrylamide, garlic acid, testis and oxidative stress.