ANTICOLITIC EFFECT OF FLAVOCOXID IN RAT EXPERIMENTAL MODEL
Nagwa I. Mohamed1*, Dalia H. El-Kashef1, Ghada M. Suddek1
1Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Mansoura, 35516, Egypt
Ulcerative colitis is a subtype of intestinal inflammatory disease characterized by up-regulation of oxidative stress and pro-inflammatory cytokines. The present study aimed to evaluate to evaluate the possible protective effect of flavocoxid in experimental colitis model in rats. Rats were randomly categorized into three groups: Control, acetic acid and acetic acid + flavocoxid groups. Flavocoxid (20 mg/kg/day) was administered orally for 7 days before induction of ulcerative colitis. On the 8th day, colitis was induced by intrarectal instillation of 2ml of 4% acetic acid using a pediatric catheter. The rats were sacrificed 24 h after acetic acid instillatiom, blood samples were collected and colons were isolated then analyzed by biochemical examination. Pretreatment with flavocoxid significantly reduced the levels of malondialdehyde (MDA) and elevated the enzymatic activity of superoxide dismutase (SOD) and level of glutathione (GSH) in colonic tissues. Our results indicate that oral administration of flavocoxid ameliorates intestinal alteration induced by acetic acid in rats via antioxidant effects. Therefore, flavocoxid might be an effective therapeutic candidate for the treatment of ulcerative colitis.