EFFECT OF MOLSIDOMINE ON LPS-INDUCED ALI
Nadeen S. Balousha, Ahmed R. El Sheakh, Ghada M. Suddek
Department of Pharmacology and Toxicology, Faculty of Pharmacy,Mansoura University, Mansoura 35516, Egypt
This study aimed to evaluate the possible modulatory effects of Molsidomine against acute lung injury (ALI) induced by LPS. In this experimental study, rats were allocated into 4 groups (n=6 per group): 1) Control; 2) LPS (10 mg/kg, I.P.); 3) LPS+Molsidomine (1 mg/kg, I.P.); 4) LPS+Molsidomine (2 mg/kg, I.P.). 24 hours later, rats were sacrificed and lung/body weight ratio was determined. Bronchoalveolar lavage fluid (BALF) and lung tissues were collected to evaluate total protein content, lactate dehydrogenase (LDH), histopathological changes and marker of oxidative stress, namely MDA. LPS-mediated ALI was evidenced in our study, as LPS significantly elevated lung/body weight ratio, BALF total protein content, BALF LDH activity and MDA levels in lung tissues. Besides LPS caused a marked histopathological damage in lung tissues. Pretreatment with Molsidomine protected against ALI induced by LPS via decreasing the elevated lung/body weight ratio, BALF total protein content, BALF LDH activity and pulmonary MDA content. Additionally, Molsidomine restored the histopathological damage induced by LPS. In conclusion, these results demonstrate that Molsidomine might possess pulmonary protective activity during endotoxemia possibly via reducing lung edema and oxidative damage.