EFFECT OF NO MODULATOR ON EXPERIMENTALLY INDUCED VASCULAR CALCIFICATION
Ahmed.Amera, Ahmed R. El-Sheakhb, Mohamed F Hamedc, Shehatou GSa,b, H El-Kashefa,b, Manar A Naderb
a Department of Pharmacology and Biochemistry, Faculty of Pharmacy, Delta University for Science & Technology, International Coastal Road, Gamasa City, Mansoura
b Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Mansoura, Mansoura.
c Department of Pathology, Faculty of Veterinary Medicine, University of Mansoura, Mansoura.
This study was designed to investigate the effect of agmatine sulfate (AGM, CAS2482-00-0) in Vitamin D3 plus nicotine (VDN)- induced vascular calcification (VC) in rats. VDN were administered to produce VC in Wistar rats with or without AGM for 4 weeks. Serum concentration of calcium and serum alkaline phosphatase (ALP) activity was detected. Aortic concentration of malondialdehyde (MDA) was determined. AGM treatment markedly reduced VDN-induced elevation in MDA content in aorta and serum alkaline phosphatase activity. However, AGM significantly increased VDN-induced reduction in serum calcium level. Therefore, agmatine successfully attenuated VC induced by VDN treatment and its antioxidant activity may contributed to this effect.