ANTIBODY ISOTYPE RESPONSES IN DIFFERENT CLINICAL PATTERNS OF SCHISTOSOMA MANSONI INFECTION
Mohamed S. Noureldin; Hassan A. El-Shinnawy*; Manar S. Azab; Mohamed A. El-Malky;
Hassan S. El-Naggar; Ayman T. Abbas* and Mohamed E. Youssef
Parasitology Department and Gastroenterology Center* Faculty of Medicine, Mansoura University
The isotypic patterns of antibodies against Schistosoma mansoni antigenic preparations from eggs (SEA), adult worms (AWA) and cercariae (CAP) have been studied in sera from one hundred and sixteen Egyptian patients. Cases were stratified into five different stages of pathology according to ultrasonographic and clinical parameters. Stage 0 included 24 patients with the chronic simple intestinal form. Stage 1 included 28 patients with the hepatointestinal form. Stage 2 included 26 patients with the hepatosplenic form. Stage 3 included 22 patients with shrunken liver and splenomegaly. Stage 4 included 16 patients with shrunken liver, splenomegaly and ascites. Antibody reactivity was measured in ELISA. This study showed that most of the cytotoxic antibodies responses against the same or different antigens were significantly correlated and showed significant positive correlation with both age and degree of disease evolution. Interestingly, IgM responses were positively correlated against different antigens examined, but showed negative correlation with age and disease stage. Most of immunoglobulin isotypes against different antigens were significantly higher in patients with evident clinical symptoms. So, IgM responses against Schistosoma mansoni antigens decrease with progress of age and diseases stage while cytotoxic antibodies responses increase. We conclude that, augmentation of immune response may be responsible for advancement in pathology and isotypic profile may predict the clinical future of cases.
December 2005